(HESA-A) in mice and rats
M. Balali-Mood
1, A. Ahmadi 2, K. Balali-Mood 3,T. Ghafghazi 4, P. Rajabi 5, M. Taher 6,Abstract
Background and Aim: HESA-A is a marine biological compound that was recently patented in the Islamic Republic of Iran, revealed anti-tumor properties in-vitro and in-vivo. The objective of this study was to investigate the acute, sub-acute, and chronic toxicity of HESA-A in mice and rats.
Materials and Methods: The acute toxicity testing of HESA-A (0.5-18 g/kg) orally in different groups of
mice and rats were undertaken. Sub-acute toxicity testing of the drug (10 g/kg/day for a week) on rats and chronic toxicity testing of different doses of HESA-A (1.25-5.00 g/kg/day for 30 days) on different groups of mice were carried out. Clinical abnormalities, changes in body temperature and weight, biochemical, haematological and pathological investigations were recorded...
Results: Acute toxic effects of HESA-A occurred after 10 g/kg and the LD50s were calculated as 16 g/kg and 18 g/kg for mice and rats, respectively. The body weight of rats taken 18 g/kg of HESA-A reduced significantly (P<0.05) at day 14 and after death. Sub-acute and chronic toxic effects of HESA-A in rats and mice were observed only in a few rats and in the mice taken 5 g/kg/day of the drug for 30 days. Drowsiness, vomiting, diarrhoea and convulsions were the common findings. The body weight of rats decreased significantly (P<0.05) from 200±21 g to 156±22 g at day 14. In few rats and in the group of mice that received 5 g/kg HESA-A daily for 30 days, mild infiltration of lymphocytes in the liver tissues were observed.
Conclusion: Based on the results, HESA-A has very little toxic effect on mice and rats.
Key Words: Toxicity; Acute; Chronic; Drug; HESA-A
1.Professor and Director, Medical Toxicology Centre, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences. Mashhad, Iran
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2.Research Physician, Cancer Research Institute, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
3.Post- Doctoral Researcher, Department of Biochemistry, Faculty of Medicine, Oxford University, England
4.Professor, Department of Pharmacology, Faculty of Pharmacy, Isfahan University of Medical Sciences. Isfahan, Iran
5.Professor, Department of Pathology, Faculty of Medicine, Isfahan University of Medical Sciences. Isfahan, Iran
6.Assistant Professor, Department of Biochemistry, Faculty of Pharmacy, Isfahan University of Medical Sciences. Isfahan, Iran
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